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Figure 4 | BMC Immunology

Figure 4

From: Tolerance induced via TLR2 and TLR4 in human dendritic cells: role of IRAK-1

Figure 4

A . Reduced TNF promoter activity in LPS tolerant dendritic cells. Day 7 dendritic cells were pre-cultured without and with LPS at 5 ng/ml and cells were infected on day 8 with the human – 1173 bp TNF promoter luciferase reporter adenovirus. On day 9 cells were stimulated for 5 hours with LPS at 500 ng/ml and luciferase activity was determined and normalized to protein content. Given is the average of 4 experiments. 100% represents 329560 +/- 373169 RLU in these experiments. * = p < 0.05 compared to 0/LPS. The hexagones denote NF-κB binding sites in the human TNF promoter at -873, a complex site from -627 to -589 including a site at – 605, and a site at -104 relative to transcription start. B. Reduced NF-κB activity in LPS tolerant dendritic cells. Day 7 dendritic cells were pre-cultured without and with LPS at 5 ng/ml and cells were infected on day 8 with a luciferase reporter adenovirus driven by a tetramer of NF-κB motifs. On day 9 cells were stimulated for 5 hours with LPS at 500 ng/ml and luciferase activity was determined and normalized to protein content. Given is the average of 3 experiments. 100% represents 13803,67 +/- 2651,93 RLU in these experiments. * = p < 0.05 compared to 0/LPS. The hexagones denote NF-κB binding sites.

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