Figure 4From: Quantitative assessment of the robustness of next-generation sequencing of antibody variable gene repertoires from immunized miceCorrelation of ranks of CDR3 and VDJ sequences between replicates is high (CDR3: r≥0.94, VDJ: r≥0.75) demonstrating reproducibility of antibody repertoire sequencing. The ranks of CDR3 (A: 1M, B: 9M) and VDJ (C: 1M, D: 9M) sequences were determined by assigning the highest rank to the CDR3 or VDJ sequence with the highest abundance, the second highest rank to the CDR3 or VDJ sequence with the second highest abundance and so forth. Association of ranks was determined using Pearson correlation (r). To circumvent overplotting, correlation plots are displayed using hexagons-purple indicates where data points accumulate. Only reliably detected CDR3 and VDJ sequences (Figure 3) were considered for the analysis shown.Back to article page