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Table 1 RSV induces cytokine release from human airway epithelial cells

From: Leukemia inhibitory factor protects the lung during respiratory syncytial viral infection

 

Airway epithelial cell type

 

SAE cells (Type I)

A549 (Type II)

Cytokines (pg/ml media)

Mock

RSV

Mock

RSV

IL-1β

12.66±1.76

31.75±1.48*

13.52±0.80

34.33±4.3*

IL-1β

9.55±1.17

15.50±2.45*

1.28±0.05

2.28±0.45*

IL1-RA

40.37±6.37

63.25±4.82*

5.20±0.37

23.28±3.88*

IL-2

0.98±0.28

1.44±0.42

0.20±0.05

0.25±0.07

IL-2RA

41.65±3.57

44.67±2.12

36.72±0.58

34.53±0.55

IL-3

102.7±8.85

100.2±7.37

59.09±3.54

58.08±4.56

IL-6

2349±234.6

3260±153.8*

17.21±0.79

534.4±19.22*

IL-7

29.36±4.87

55.37±8.32*

17.00±4.57

34.44±4.15*

IL-8/CXCL8

2479±154.4

5094±363.5*

142.2±20.70

1203±83.75*

IL-10

6.89±1.31

8.13±0.15

5.85±0.27

7.02±0.24*

IL-12(p40)

193.4±17.18

183.9±19.78

127.2±10.11

135.0±19.54

IL-12(p70)

36.76±5.48

36.09±5.94

14.54±1.29

19.86±0.81*

IL-16

115.7±14.5

223.5±19.04*

652.1±32.79

387.5±45.97

IL-18

6.70±0.71

11.94±0.92*

6.24±0.31

7.06±0.37

GROα/CXCL1

8482±428

11119±639*

2431±177.7

9473±366.4*

Eotaxin/CCL11

5.16±0.24

10.37±0.62*

1.38±0.16

3.43±0.37*

FGF

6.60±0.30

10.72±1.41*

4.24±0.51

13.92±2.16*

G-CSF/CSF-3

945.6±72.01

1403±130.2

1.00±0.79

10.16±1.57*

GM-CSF/CSF-2

9.74±2.99

10.04±3.15

3.73±0.44

7.12±0.51*

M-CSF/CSF-1

73.03±8.60

98.50±6.41

2.90±0.74

4.55±0.63

IFN-γ2

4.62±0.32

4.97±0.27

4.46±0.04

4.55±0.76

IFN-γ

95.39±13.08

171.6±12.04*

12.38±1.98

81.81±8.88*

MIG/CXCL9

29.01±3.91

61.41±2.99*

53.83±5.43

104.81±12.11

IP10/CXCL10

337.6±93.99

10580±546*

5.13±0.35

1836±97.5*

MCP-1/CCL2

146.2±15.51

266.0±42.71*

557.1±30.48

1088±56.54*

MCP-3/CCL7

71.68±8.97

215.8±17.44*

96.03±10.57

78.41±11.14

MIP-1β/CCL3

1.88±0.12

2.38±0.36

1.25±0.09

16.96±0.42*

MIP-1β/CCL4

1.55±0.32

2.75±0.45

0.77±0.13

89.04±7.52*

MIF

2123±186.2

7435±179.5*

454.0±20.8

1015±47.5*

PDGF-BB

6.91±1.01

16.90±2.98*

3.68±0.59

10.23±0.16*

RANTES/CCL5

206.8±10.32

339.9±29.54*

44.30±8.53

656.9±82.5*

TNF-α

16.78±0.57

32.40±3.27*

1.14±0.12

27.83±2.37*

TNF-α/LTA

21.98±1.39

21.09±0.78

17.59±0.18

17.41±0.72

VEGF

3717±372.2

5256±304.3*

1585±108.9

3297±146.2*

TRAIL

135.7±13.64

209.2±6.82

151.4±10.16

191.9±5.06*

β-NGF

23.92±1.76

23.81±2.23

20.50±1.17

24.12±2.39

LIF

114.9±11.92

227.4±6.7*

64.70±2.41

212.7±15.88*

HGF

63.33±4.98

81.84±1.41*

71.23±1.47

69.34±1.71

CTAK/ CCL27

130.5±9.87

249.3±9.73*

122.5±2.40

211.7±8.91*

SDF-1α/CXCL12

672.0±31.7

1504±97.33*

121.6±9.61

359.4±28.6*

SCGF-β

685.7±32.1

1000±73.1*

3575±191.2

5905±161.9*

SCF

19.09±1.08

42.87±0.18*

27.50±1.52

38.66±3.03*

  1. Cytokine levels were determined in cell culture supernatants from A549 or SAE cells 24 hours post RSV challenge, by multiplex analysis. Values are represented as mean media concentration (pg/ml)±S.E.M. Assays were performed in triplicate from samples collected on separate days, where n = 10 samples/group. Bold numbers denoted by *represents a p value less than 0.05 compared to mock treated cells.