Figure 5

Glomerulonephritis in RIIB^−/−, SLAM¹²⁹, and RIIB^−/−SLAM¹²⁹mice. (A) Kidney sections from each mouse line at week 45 were stained with HE (upper) and PAS (lower) for examination of glomerular disease, which was assessed according to the criteria depicted in Methods. Original magnification, ×200. Scale bar, 100 μm. (B) Comparison of the disease scores among RIIB^−/−, SLAM¹²⁹, RIIB^−/−SLAM¹²⁹ and B6 mice at 45 weeks of age. F, female; M, male. Horizontal bars denote the mean values, excepting the plot of RIIB^−/−SLAM¹²⁹ male for single determination. ***P <0.001 (n =3-6). For scoring, ≥25 glomeruli were examined for each line. Student’s t-test. (C, D) For IgG-immune complexes (IgG-ICs) deposition, kidney sections from each mouse line at week 45 were stained with FITC-anti mouse IgG. Original magnification, ×200 (C) or × 100 (D). Scale bar, 10 μm. The figure is representative of most of the glomeruli observed in three mice per group.