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Figure 1 | BMC Immunology

Figure 1

From: Multiple factors influence the contribution of individual immunoglobulin light chain genes to the naïve antibody repertoire

Figure 1

Vκ10 quantitative RT-PCR. (A) BALB/c IgM−/CD43−/B220+ bone marrow cells and (B) spleen. A maximum of two μg RNA was used as template for RT reactions with oligo d(T) or Cκ primers. One tenth of each cDNA reaction was used as template in qPCR with primers that amplify all Vκ10 genes equally. Gene-specific probes were used in each reaction to identify Vκ10A, B or C. Vκ10A, B and C standard templates were used as cross-probe controls in each experiment and dilutions were used to construct standard curves. The level of Vκ10 target in each sample was calculated by interpolation into the standard curve. Results are expressed as femtograms (fg) of detected Vκ10 target/μg of total RNA in the RT reaction.

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