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Figure 3 | BMC Immunology

Figure 3

From: Multiple factors influence the contribution of individual immunoglobulin light chain genes to the naïve antibody repertoire

Figure 3

Vκ10 light chain pairing with heavy chain. (A) Alignment of the Vκ10AJκ1, Vκ10BJκ1 and Vκ10CJκ1 amino acid sequences derived from rearrangements amplified by PCR and cloned separately into the phagemid vector pCES (Cκ is not shown but is identical for all). (B) VH genes amplified from pre-B cells and (C) VH genes amplified from splenic B cells. VDJ-CH1 rearrangements were amplified from cDNA derived from total RNA of LPS-stimulated splenic B cells (two mice separately), sorted pre-B cells (pooled from 5 mice) and from pre-B cells expanded ex-vivo on OP-9 stromal cells stimulated with IL-7. Phage expressing both μ and κ were selected by sequential panning on anti-κ and anti-μ coated surfaces and sequenced. The genomic linear order of VH gene families from five prime to three prime is shown on the bottom of each graph. Bars with diagonal lines and gray stippled bars represent the number of germ line VH sequences in each VH family for C57BL/6 and 129S1 mice, respectively. White, gray and black bars show the number of unique VH sequences isolated for each VH family that paired with Vκ10A, Vκ10B and Vκ10C, respectively.

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