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Figure 5 | BMC Immunology

Figure 5

From: Multiple factors influence the contribution of individual immunoglobulin light chain genes to the naïve antibody repertoire

Figure 5

VκJκ-iRS receptor editing of Vκ10 productive and nonproductive rearrangements. (A) Schematic of the kappa locus (not drawn to scale) depicting VκJκ-iRS recombination products resulting from recombination of the RS element with an isolated heptamer in the Jκ-Cκ intron. Arrows represent the PCR primers used to amplify the edited Vκ10Jκ rearrangement. The upstream primer anneals in Vκ10 framework 1 to a sequence that is identical for all Vκ10 genes. The downstream primer anneals to a sequence in the RS. (B) Percent of productive and nonproductive recombination products of RS edited Vκ10A, Vκ10B and Vκ10C rearrangements to Jκ1, Jκ2, Jκ4 and Jκ5.

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