Figure 4

Human PBLs transduced with the EpCAM specific CAR inhibit PC3M tumor growth in vivo . A) A diagram of the experimental design. NOD/SCID mice were injected intraperitoneally with 5 × 10⁵ PC3M-luc cells on day 0 and tumor growth was examined by luminescence imaging on day 4. On day 5, PBLs transduced with the EpCAM-specific CAR or control vector were injected intravenously at 1 × 10⁷ cells/mouse and the anti-tumor effects were determined by luminescence imaging on days 8 and 11. B) Representative luminescence images of PC3M-luc tumor-bearing mice before and after PBL treatments. Tumor-bearing mice without treatment were used as controls. C) Quantitative results of the luminescence intensity of PC3M-luc tumor-bearing mice before and after PBL treatment. Statistical analysis was performed using a two-way analysis of variance.