Fig. 4
Modulation of chemokine-mediated chemotaxis by B and T lymphocytes from propolis-treated diabetic mice. PBMCs were subjected to migration assays in response to CCL21 and CXCL12. a Representative dot plots showing the gating strategy to obtain viable lymphocytes based on forward and side scatter and to discriminate between the CD45R/B220+ cell population (B-lymphocytes) and the CD45R/B220neg cell population (T-lymphocytes) using a PE-CD45R/B220 mAb and flow cytometric analysis. The input cells and the migrated cells were stained with the PE-CD45R/B220 mAb. The cells were then counted for 60 seconds via flow cytometry to calculate the percentage of cells that migrated nonspecifically (based on the number of cells that migrated to medium alone) or specifically (based on the number of cells that migrated to medium containing a chemokine). To calculate the percentage of specific migration induced by chemokines, the percentage of cells migrating to medium alone was subtracted from the percentage of cells migrating to the medium containing a chemokine. b & c The data from the different experiments (n = 10) are expressed as the mean percentage of chemokine-mediated specific migration of B and T lymphocytes ± SEM in control mice (open bars), diabetic mice (black bars) or propolis-treated diabetic mice (hatched bars). *P < 0.05 for diabetic versus control; #P < 0.05 for diabetic + propolis versus diabetic; +P < 0.05 for diabetic + propolis versus control (ANOVA followed by Tukey’s post-test)