Fig. 7
From: In silico prediction of Ebola Zaire GP1,2 immuno-dominant epitopes for the Balb/c mouse
CTL response induced by GP1,2 immunization. Splenocytes from immunized animals were cultured for 5 days in vitro with P8, P9 and P10 peptides, for the expansion of peptide specific CTLs, that were collected and counted. In parallel, KD cells were incubated overnight with P8, P9, P10 or adjuvant (mock control), washed and then labeled with DDAO cell tracker. Effector and target cells were mixed and incubated for 4 h in the indicated ratios and fixed. The cells were then stained with anti-cleaved Caspase-3 PE and analyzed by flow cytometers, using a FACS Canto. The mean percentage of Caspase positive KD cells +/− the SE is presented. The results are representative of three independent experiments. Asterisks indicate statistically significant increase in Caspase -3 positive cells compared with those Caspase-3 positive cells in mock (* = p < 0.05)