Fig. 8
From: In silico prediction of Ebola Zaire GP1,2 immuno-dominant epitopes for the Balb/c mouse
Induction of intracellular IFN-γ production following immunization with GP1,2-Fc. Splenocytes from GP1,2-Fc immunized animals were cultured for 24 h in vitro with P8, P9 and P10 peptides. Cells were collected, washed, stained with CD3ε PerCP and anti-CD 8 FITC and fixed. The cells were further stained with anti-IFN-γ antibody and were analyzed using BD FACS Canto. The results are represented as mean +/− S.E. of two independent experiments