Fig. 3

Decreased MIP-2 production and normal neutrophil responses in Dcir1 ^−/− mouse colitis colon. a Chemokine and cytokine production in organ cultures was analyzed. Tissue fragments of ceca and colons from 5 days DSS-treated mice were cultured for 24 h, and chemokine and cytokine production was measured by CBA or ELISA. Data are the combined results of two independent experiments (n = 6). *P < 0.05. b Chemotactic activity of neutrophils to MIP-2 was assessed using transwells. After staining with FITC-anti-Ly6G and PE-anti-CD11b, bone marrow cells that migrated into the lower wells in the presence of 100 ng of MIP-2 were analyzed by flow cytometry after 1 h (left panels). Data are representative of two independent experiments (n = 4) (right bar panel). Data are expressed as the mean ± SEM