Fig. 2

Expression levels of IgA in the gastrointestinal tract of mice post infection with T. gondii PRU. Quantification of IgA⁺ cells in a: small intestine and b: large intestine of BALB/c and ΔdblGATA-1 mice following infection with T. gondii PRU. BALB/c and ΔdblGATA-1 naïve mice (n = 6; combined from 2 independent experiments), 10 days post-infection (n = 7-9; combined from 2 independent experiments) and 13 days post-infection (n = 4). c-f. representative photos of immunohistochemistry in naïve and at day 10 post infection in small intestine tissue from BALB/c and ΔdblGATA-1 mice (g). Quantification of CD138⁺B220^lo plasma cells in the mesenteric lymph nodes of naïve and infected mice (n = 3-5) and (h). brain parasite burden expressed as relative expression of bradyzoite amplification compared to murine housekeeping gene *p < 0.05, **p < 0.01, ***p < 0.001. Two-way ANOVA with post-hoc Bonferroni Scale Bar represents 100 μm. Arrowheads in inset images indicate positive cells