Fig. 5
From: Active immunization with human interleukin-15 induces neutralizing antibodies in non-human primates
Recognition and neutralizing capacity of sera from the Alum group in presence of simian IL-15. a ELISA for recognition of simian IL-15 by Abs from sera of immunized macaques. The plate was coated with 1 μg/ml recombinant simian IL-15 and it was incubated with the pool of sera per group diluted 1:4000. b Inhibition of simian IL-15-induced proliferation of CTLL-2 cells by sera from the Alum group. Cells were cultured in the presence of simian IL-15, simian IL-15 plus serial dilutions of serum (starting dilution 1:25) or medium. Cell proliferation was evaluated by MTT staining. All tested sera correspond to 15 days after the third immunization