Fig. 1

Kinetics of cytokine production. Milliplex kits from Millipore (see Material and Methods for details) were used to perform kinetic experiments with PBMC from two healthy donors stimulated for 1, 2 or 5 days with PPD (1 μg/ml) and SEB (10 ng/ml) as positive control. Non-stimulated (NS) PBMC were used as negative control. Results obtained for samples from donor 1 (IL-2 and IL-17) and donor 2 (IFNγ and IL-5) are shown. NS and PPD values correspond to the left y axis and SEB values to the right y axis