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Table 2 Intraclass correlation coefficients (ICCs) across HIV-stimulated samples

From: Optimization and evaluation of Luminex performance with supernatants of antigen-stimulated peripheral blood mononuclear cells

Cytokine

 

FI

Conca (pg/ml)

Conc in range (pg/ml)

Culture duration

Samples (n)

Total meas. (n)

ICC

Samples (n)

Total meas. (n)

ICC

Samples (n)

Total meas. (n)

ICC

IFNγ

11d

16

114

.69

16

107

.11

16

81

.51

Perforin

11d

16

91

.66

16

71

.17

16

61

.72

IL-5

11d

16

114

.36

16

106

.25

16

99

.23

IL-17

11d

16

114

.02

16

69

.02

16

28

.19

IFNγ

2d

17

135

.93

17

129

.88

17

122

.81

Perforin

2d

17

111

.16

17

97

NA

17

95

.88

IL-5

2d

17

135

.58

17

91

.55

17

86

.53

IL-17

2d

17

135

.43

17

37

.29

1

1

-

  1. ICCs were calculated across 9 replicate cultures. The combination ‘donor x stimulation’ was treated as one sample. d day, Conc concentration, meas measurement. ICCs > 0.8 are indicated in bold
  2. aincluding extrapolated values, NA: G matrix ‘not positive definite’