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Table 4 Inter-assay variability of internal control

From: Optimization and evaluation of Luminex performance with supernatants of antigen-stimulated peripheral blood mononuclear cells

Plates

IL-17F

GM-CSF

IFNγ

IL-10

CCL20/MIP3α

IL-13

IL-17A

IL-22

IL-9

IL-2

IL-5

IL-27

TNFα

1

2091.2

7423.2

5300.1

81.4

9523.6

2248.3

74.0

45.4

225.6

89.8

191.9

808.9

3424.9

2

2059.3

6734.8

6833.8

82.0

9009.3

2537.1

90.9

65.0

256.9

107.0

224.2

1081.6

4126.7

3

1963.3

6811.0

5144.7

74.7

5706.4

2268.3

82.2

46.3

216.1

90.9

214.7

786.4

3571.6

4

2215.3

8326.4

5348.7

78.4

8241.1

2412.0

83.1

53.8

231.1

104.7

205.1

1308.3

3953.4

5

1998.1

6735.9

5506.4

61.1

8311.7

2064.6

72.1

48.5

195.9

89.2

172.9

1026.8

3385.1

6

2238.5

7407.3

6040.5

85.1

6113.7

2259.9

89.1

61.5

263.3

84.2

218.3

1093.6

4302.2

7

2473.7

8788.0

5809.2

71.3

8211.6

2560.0

88.3

47.0

278.8

98.5

243.6

1102.3

4128.4

8

2425.1

7440.4

5180.8

81.6

10147.7

2429.5

84.7

50.8

254.4

105.9

214.3

1190.2

4310.1

9

1945.9

6719.6

4545.8

68.4

8901.7

2384.8

82.4

53.3

198.0

95.8

221.2

1175.7

4241.0

10

2165.8

7817.6

7062.7

88.5

10767.6

2729.8

87.8

50.3

296.4

110.3

241.2

1070.5

5285.1

11

2185.4

6660.9

4907.7

68.6

8756.7

2387.7

70.6

39.6

180.1

91.9

170.5

914.5

3409.2

12

2151.4

7223.0

5796.1

65.0

8429.9

2304.6

71.1

42.6

224.8

86.8

195.3

1088.9

4515.4

13

2231.2

7964.7

5324.4

78.5

7028.9

2618.0

87.1

51.7

237.8

113.9

230.2

1249.2

4951.5

%CV

7.4

9.2

12.8

11.0

17.2

7.4

9.0

14.0

14.4

10.0

11.0

14.6

14.3

  1. Mean concentration values of duplicate Internal Controls (pg/ml) and %CVs of mean concentration calculated using the 13 inter-plates internal control mean concentration values for the selected 15-plex Millipore magnetic kit (see Material and Methods section for detailed analytes). IL-23 and IL-1β %CVs were not calculated due to concentration values < LLOQ and > ULOQ, respectively