Antigen presenting capacity of murine splenic myeloid cells

BMC Immunology

Table 1 Phenotypic identification of myeloid and dendritic subsets in spleen

Subsets	Phenotype^a
CD8⁺ cDC	CD11b⁻CD11c^hiCD8⁺CD43⁻Ly6C⁻Ly6G⁻MHCII⁺
CD8⁻ cDC	CD11b⁺CD11c^hiCD8⁻CD43⁻Ly6C⁻Ly6G⁻MHCII⁺
L-DC	CD11b^hiCD11c^loCD8⁻CD43⁺Ly6C⁻Ly6G⁻MHCII⁻Siglec-F⁻
Resident monocytes	CD11b^hiCD11c^loCD8⁻CD43^hiLy6C⁺Ly6G⁻MHCII⁻Siglec-F⁻
Inflammatory monocytes	CD11b^hiCD11c⁻CD8⁻CD43⁺Ly6C^hiLy6G⁻MHCII⁻Siglec-F⁻
Eosinophils	CD11b^hiCD11c⁻CD8⁻CD43⁺Ly6C⁺Ly6G⁻MHCII⁻Siglec-F⁺
Neutrophil	CD11b^hiCD11c⁻CD8⁻CD43⁺Ly6C⁺Ly6G⁺MHCII⁻Siglec-F⁻

^a Splenocytes were prepared by removal of red blood cells and T and B lymphocytes, and then cells stained with cocktails of antibodies for separation of subsets and sorting using flow cytometry. The phenotype of myeloid subsets was delineated previously in Hey et al. [10]. The phenotype of cDC subsets was taken from Merad et al. [56]

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