Fig. 2From: Effect of cryopreservation on delineation of immune cell subpopulations in tumor specimens as determined by multiparametric single cell mass cytometry analysisDetection of cellular subsets in PBMC samples by mass and fluorescent cytometry. a Representative gating scheme identifying major immune cell populations in PBMCs by FC and MC. Singlet cells, deemed viable by a Live/Dead marker (FC) or DNA intercalator (MC) were used as the parent population for cell surface marker analysis. Percentage of positive cells on a bivariate plot of CD45 and markers common to both platforms were compared. Markers included in analysis: CD11b, CD127, CD14, CD15, CD19, CD25, CD27, CD3, CD4, CD86, CD8a, HLA-ABC, HLA-DR, PD-1 and PD-L1. b Comparison of population percentages quantified by FC and MC. Percentages of cells positive for CD45 and 15 common markers were quantified by both platforms. Data represents log10 (average) ± standard deviation (SD) (N = 3) of percent positive cells. Correlation between FC and MC was determined by Pearson Product Moment Correlation (PPMC) (r = 0.96, p < 0.0001)Back to article page