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Fig. 4 | BMC Immunology

Fig. 4

From: Effect of cryopreservation on delineation of immune cell subpopulations in tumor specimens as determined by multiparametric single cell mass cytometry analysis

Fig. 4

MC analysis of expression patterns of immunomodulatory and disease prognostic biomarkers in immune and tumor cell subsets. ViSNE analysis of fresh renal cell carcinoma performed using single nucleated cells as top level population. A total of 40,000 cells was analyzed and clustered using the following markers: CD45, CD19, CD11B, CD4, CD8A, CD11C, CD34, CD66B, CD14, CD15, CD3 and CD56. The cells in the ViSNE map are colored according to the median intensity of expression for markers as identified in the top left corner of each figure. a Immunophenotyping of CD45+ cells in solid tumor sample. T-cell subsets are identified by expression of CD4 and CD8 markers, B-cells by expression of CD19, NK cell by CD56+, DC cells by CD11C+, Monocytes CD14+, Neutrophils CD15+, and MDSC by CD15+/CD66B+. Checkpoint regulatory receptors are represented by inhibitory (PD-1, BTLA, CTLA-4, TIM-3, and TIGIT) and stimulatory (GITR, CD137, CD27) markers on identified cellular subsets. b Immunophenotyping of CD45- cells in solid tumor sample. Specific subsets (Sp) were identified based on these expression patterns; Sp1: CD34bright, CD107a+, HLA-ABC+, HLA-DRmid. Sp2: CCR9+, CD56+, CTLA-4+, PD-L1+, PD-L2+

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