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Fig. 3 | BMC Immunology

Fig. 3

From: Peptides mimicking viral proteins of porcine circovirus type 2 were profiled by the spectrum of mouse anti-PCV2 antibodies

Fig. 3

Localization of viral proteins of PCV2 by indirect IFA. Localization of viral proteins of PCV2 was assessed by indirect IFA using anti-PCV2 polyclonal antisera on the Porcine Circovirus Type 2 FA substrate slide (VMRD). Each column represents a different serum staining (a, f, and k) SPF mouse serum, (b, g, and l) anti-C3 mouse serum, (c, h, and m) anti-N2 mouse serum, (d, i, and n) anti-VLP of PCV2 mouse serum, (e, j, and o) PCV2 convalescent-phase swine antiserum. Upper row: Fluorescence microscopy of viral proteins of PCV2 were identified (green). Middle row: Nuclei were stained with DAPI (blue). Bottom column: The merge of the images. Scale bars, 20 μm. b, c, and d One representative image from a single experiment with a total of four mouse sera was shown. e One representative image from of a single experiment with a total of four pig sera was shown

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