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Fig. 1 | BMC Immunology

Fig. 1

From: The immunoregulatory role of alpha enolase in dendritic cell function during Chlamydia infection

Fig. 1

Dynamic Distribution of ENO1 in DCs. a Dendritic Cell lysates were extracted from Chlamydia pulsed or nonpulsed WT DCs and IL-10−/− DCs. The Cy5 represents red dye and Cy3 represents green dye. The red color indicates high expression of protein in IL-10−/− DCs while the green color indicates high expression of protein in WT DCs. The yellow color indicates that the protein expression is the same for WT and IL-10 −/− DCs. b Western blot analysis of WT DCs and IL-10−/− DCs pulsed for 0 h, 1 h, and 2 h with Chlamydia. This result shows that ENO1 expression is decreased in WT DCs pulsed with Chlamydia. However, ENO1 expression is highly expressed in IL-10−/− DCs pulsed with Chlamydia. c In addition, we compared the amount of Chlamydia pulsed and nonpulsed WT and IL-10−/− DCs expressing ENO1 using confocal microscopic analysis. There was a significant difference in the amount of ENO1 expressing DCs in IL-10−/− DCs and WT DCs (p < 0.05). * Denotes statistical significance. We repeated this experiment 3 times

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