Fig. 2

ENO1 Knockdown with ENO1-set siRNA Lentivector. a The confocal microscopy shows that that ENO1 is expressed most abundantly in the cytoplasm of the DCs. b ENO1 siRNA was used to knockdown ENO1 in WT DCs at 0 h, 24 h and 48 h. Scramble siRNA was used as a control for ENO1 knockdown confirmation. ENO1 is expressed in siRNA Scramble control and ENO1 siRNA at 0 h and 24 h respectively. ENO1 expression was completely knocked down after 48 h of transfection. Therefore, we used WT cells, which have been transfected with ENO1 siRNA for 48 h throughout our experiments to analyze the effects of ENO1 on DC function. We repeated this experiment 3 times