Fig. 5

ENO1 regulates DC maturation and activation (1). a Flow cytometry analysis using BD Accuri C6 analyzer was used to determine TLR4 expression. DCs were stained with CD11c PE-CY7 stain and gated for further analysis. Cells were then stained with FITC conjugated with TLR4. TLR4 expression was significantly lower in Chlamydia pulsed ENO1 knockdown DCs in comparison to Chlamydia pulsed WT DCs. b Flow Cytometry analysis was also used to determine the population of cells with the maturation and activation markers CD40 and CD86 expression in DCs. Cells were stained with FITC conjugated CD40 and PE Conjugated CD86. Samples double stained with PE and FITC were compensated. Green-B-Fluorescence indicates FITC staining and Yellow-B Fluorescence indicates PE staining. c The graph shows that there were significant differences between WT and ENO1 knockdown DCs (p < 0.05). The expression of CD86 was lower in Chlamydia pulsed ENO1 knockdown DCs compared to WT DCs. While, CD40 expression increased in Chlamydia pulsed ENO1 knockdown DCs. Experiment was repeated 3 times for each sample. * Denotes statistical significance