Fig. 7
From: The immunoregulatory role of alpha enolase in dendritic cell function during Chlamydia infection
ENO1 regulates DC maturation and activation (3). a Bio-Plex Pro Mouse Cytokine 23-Plex multiplex array (Bio-Rad, Hercules, CA) was used for cytokine analysis according to manufacturer’s guidelines. The expression of Th1 type cytokines (IL-1α, IL-1β, IL-12, and IFN-y) were low (p ≤ 0.05), while, there was a high expression of the Th2 cytokines IL-4, IL-10, and IL-17 in Chlamydia pulsed ENO1 knockdown DCs compared to Chlamydia pulsed WT DCs (p ≤ 0.05). TNF-α was highly expressed in ENO1 knockdown DCs (p ≤ 0.05). Experiment was repeated 3 times. b Chemokine analysis showed that Chlamydia pulsed ENO1 knockdown DCs have low expression of monocyte chemoattractant protein 1 (MCP-1) and Rantes and high expression of MIP-1α and MIP-1β compared to Chlamydia pulsed WT DCs. * denotes statistical significance. Experiment was repeated 3 times