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Fig. 8 | BMC Immunology

Fig. 8

From: The immunoregulatory role of alpha enolase in dendritic cell function during Chlamydia infection

Fig. 8

Effect of ENO1 on the antigen presenting function of DCs in vivo and in vitro. a The T-cells collected from the spleen of Chlamydia infected WT mice were co-cultured with WT and ENO1 knockdown DCs, and their supernatant was collected for cytokine analysis. Proliferation assay was also performed to determine T cell activation. Data showed that ENO1 knockdown DCs had lower levels of antigen specific proliferation of T cells (p ≤ 0.05). * Denotes statistical significance. Experiment was repeated 3 times. b Results showed that there was a decrease in IL-1α, IL-1β, & Rantes secretion and an increased secretion of IL-5, IL-13, IL-17A, and IL-10 in Chlamydia pulsed ENO1 knockdown DCs incubated with immune CD4+ T cells (P ≤ 0.01). * Denotes statistical significance. Experiment was repeated 3 times. c We adoptively transferred WT and ENO1 knockdown DCs into naive WT mice through tail vein injection. The mice were then infected intravaginally with 105 IFU of C. muridarum 1 week after adoptive transfer. The course of infection and bacterial load were monitored. WT mice that received Chlamydia pulsed ENO1 knockdown DCs experienced a longer period of bacterial shedding compared to the Chlamydia pulsed WT DCs and IL-10−/− (KO). * Denotes statistical significance

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