Fig. 2

Phosphosite identification within tyrosine-protein kinase SYK. (a) Sequence coverage of murine SYK. Residues which were not sequenced are highlighted in grey, and phosphorylated residues are highlighted in black. MS2 spectral identification information can be found in the Additional files. The localization of phosphosites relative to protein tertiary structure are shown in (b). Phosphosites which were subsequently utilized for MRM quantitation are shown in red font. (c) Assignment of phosphosites to treatment classes. Blue shaded boxes represent phosphosites that have been identified in homologous sequences of human (Hu) recombinant SYK [34]. Black shaded boxes indicate that a spectral determination was positively identified for the indicated phosphosite and treatment regimen at any dosage tested. NT, not treated; IgM, activating anti-IgM antibody; Hg, mercury; PV, pervanadate