Fig. 1From: Blood handling and leukocyte isolation methods impact the global transcriptome of immune cellsSimulated sample handling methods. Diagram outlines methods used to simulate various sample handling techniques representative of those encountered in clinical immunology, including: blood shipping temperature (20 °C or 4 °C), peripheral blood mononuclear cell isolation method (Ficoll gradient, collagenase plus percoll gradient, whole blood lysis, leukocyte filtration), and immune cell types of interest (CD8+ T cells, CD4+ T cells, granulocytes or monocytes)Back to article page