Reverse primer used for the amplification of heavy chain variable regions | Reverse primer used for the amplification of κ light chain variable regions |
 R1: AGGATCCAGGRRCCARKGGATAGACAGATG | R2: AGGAGATGGTGGGAAGATGGATACAGTTG |
Degenerate primer used for the amplification of heavy chain variable regions | Degenerate primer used for the amplification of κ light chain variable regions |
hF: | κF: |
 1. GCCCGTTTCTGCTAGC ATGTTGGTSCTGMAGTKGGTTTTSGTG | 1. GCAATCCAGGTCCA ATGAAAATGACGACACCTGCTCAGTTC |
 2. GCCCGTTTCTGCTAGC ATGAGACTACTAGGTCTTCTCCTGTGC | 2. GCAATCCAGGTCCA ATGAGGGCCCCTTTTCAGTTACTTGGG |
 3. GCCCGTTTCTGCTAGC ATGGGATGGAGCCAGATCATCCTCTTT | 3. GCAATCCAGGTCCA ATGTCTAAAAACTTATTAGAAGTTTCA |
 4. GCCCGTTTCTGCTAGC ATGGTTTTCGGGCTGATCCTTTTTCTT | 4. GCAATCCAGGTCCA ATGGTAAGTCCTGCCCAGTTCCTGTTT |
 5. GCCCGTTTCTGCTAGC ATGATGGGTTTAGGGGTGATTCTTTTT | 5. GCAATCCAGGTCCA ATGATGGCTGCAGTTCAACTCTTAGGG |
 6. GCCCGTTTCTGCTAGC ATGGGATTGAGCTGGGTTTTTCTTGTT | 6. GCAATCCAGGTCCA ATGAAAGTGCCTGGTAGGCTGCTGGTG |
 7. GCCCGTTTCTGCTAGC ATGGACTTGCGACTGACTTATGTCTTT | 7. GCAATCCAGGTCCA ATGAAGTGGCTGTTAGTCTGTTGGTGC |
 8. GCCCGTTTCTGCTAGC ATGACTATCCTGGTGCTTCTCCTCTGT | 8. GCAATCCAGGTCCA ATGATTCCTGCCCAGTTCCTGTTTCTG |
 9. GCCCGTTTCTGCTAGC ATGGCTRTCCTGGTGCTGTTGCTCTGC | 9. GCAATCCAGGTCCA ATGATGAGTCCTGCCCAGTTCCTGTTT |
 10. GCCCGTTTCTGCTAGC ATGRAATGSARCTGGRTCWTYYTCTTY | 10. GCAATCCAGGTCCA ATGAATTTTCAGGTGCAGGTTTTTAGC |
 11. GCCCGTTTCTGCTAGC ATGGCTGTCCTGGTGCTGTTGCTCTGC | 11. GCAATCCAGGTCCA ATGAAYGTGYCCACTCAACTCCTTGGG |
 12. GCCCGTTTCTGCTAGC ATGAGARYGYYGRKTCTTCTGTACCTG | 12. GCAATCCAGGTCCA ATGGACATGAGGGCCCATRCTCAGTTT |
 13. GCCCGTTTCTGCTAGC ATGGACWTCAGNCTCAGCTTGGBTTTC | 13. GCAATCCAGGTCCA ATGARRKTYSNBVYTSAGYTTYKKGGG |
 14. GCCCGTTTCTGCTAGC ATGRAGTTGDGSMTRANCTGGRTTTTY | |
Primer used for the amplification of heavy and light chains | |
 F3: CATCTGTCTATCCACTGGCTCCTGGAAC | R3: TGACAGGTGCGCGTTTAGCACG TCATTTACCCGGAGAGTGGGAGAG |
 F4: CGTGCTAAACGCGCACCTGTCAAAC | R4: TGGACCTGGATTGCTTTCTACATC |
 F5: CAACTGTATCCATCTTCCCACCATC | R5: GATCCGGCCTTGCCGGC CCTAACACTCATTCCTGTTGAAG |