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Fig. 2 | BMC Immunology

Fig. 2

From: Human NK cells adapt their immune response towards increasing multiplicities of infection of Aspergillus fumigatus

Fig. 2

Expression of cytokine mRNA after 3 and 6 h of stimulation analyzed by conventional PCR. Left side: Freshly isolated NK cells were pre-incubated overnight with pro-leukin and stimulated with IL-2 / Il-15 (IL-15) or A. fumigatus (Afum) germ tubes for 3 h. Unstimulated controls were included (−/−). RNA was isolated and used for RT-PCR detection of CCL4/MIP-1β, CCL3/MIP-1α, and XCL1/lymphotactin expression. PCR products were analyzed by gel electrophoresis. Right side: The experiment was performed three times in total for 3 (upper row) and 6 h (lower row) of stimulation, as indicated. Using ImageJ, the relative color intensity of each DNA band was quantified as a measure for the initial expression rate of the appropriate mRNA. Means and standard deviations were summarized in diagrams, *p < 0.05

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BMC Immunology

ISSN: 1471-2172

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