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Table 1 Primers for qPCR analysis

From: Human NK cells adapt their immune response towards increasing multiplicities of infection of Aspergillus fumigatus

Gene

Forward

Reverse

Product size (bp)

ALAS1a

GGCAGCACAGATGAATCAGA

CCTCCATCGGTTTTCACACT

150

CCL3 (MIP-1α)

TGCAACCAGTTCTCTGCATC

TTTCTGGACCCACTCCTCAC

198

CCL4 (MIP-1β)

GCTTCCTCGCAACTTTGTGG

TCACTGGGATCAGCACAGAC

111

CCL5 (RANTES)

TCATTGCTACTGCCCTCTGC

TACTCCTTGATGTGGGCACG

115

CXCL8 (IL-8)

GGTGCAGTTTTGCCAAGGAG

TTCCTTGGGGTCCAGACAGA

183

XCL1 (lymphotactin)b

CTCCTTGGCATCTGCTCTCT

CCTTCCGTGATGGTGTAGGTC

137

IFNG (IFN-γ)c

GCATCCAAAAGAGTGTGGAG

GCAGGCAGGACAACCATTAC

255

TNF (TNF-α)

TGCTTGTTCCTCAGCCTCTT

TGGGCTACAGGCTTGTCACT

185

CSF2 (GM-CSF)

GCCCTGGGAGCATGTGAATG

CTTGTAGTGGCTGGCCATCAT

223

IL1A (IL-1α)

TGATCAGTACCTCACGGCTG

TGGTCTTCATCTTGGGCAGT

156

IL16 (IL-16)

CGAAGACTCAGCTGCAAATGG

GCAGGGAGATAACGGACTGAC

167

GZMB (granzyme B)

TGCGAATCTGACTTACGCCA

GCATGCCATTGTTTCGTCCA

160

  1. aALAS1 is well established as a housekeeping gene for the normalization of gene expression by cells confronted with different stimuli [63,64,65,66,67]. In general, primers were designed to include as much transcript variants of the mentioned gene as possible. Within this context, bprimers for XCL1/lymphotactin qualify also for the amplification of XCL2, which differs just slightly with regard to the amino acids, but show the same functionality in vitro [68]. cPrimers for IFNG were already published by Bouzani et al. [27]