Fig. 2
Neutrophil-dominated inflammation in the airways of βENaC-Tg mice at 6 and 8 weeks. Flow cytometry was used to measure myeloid cell populations collected from the BAL of mice at (a) 6 and (b) 8 weeks. There were significantly more myeloid cells, and neutrophils per 1000 cells in the BAL of βENaC-Tg mice at 8 weeks, as compared to wild-type mice. Statistical significance was determined with a Two-way ANOVA (n = 6, **, p < 0.05, ***, p < 0.001, ****, p < 0.0001). c Cytospin preparations of BAL from representative WT or βENaC-Tg mice were fixed and stained to visualize the cellular makeup of the BAL. d Immunofluorescence staining of 1.5 × 105 cells from 1 to 2 mice per group shows neutrophils in the BAL of 8 week-old βENaC-Tg mice through detection of mouse myeloperoxidase (MPO, orange) and DNA (blue, DAPI). e Immunohistochemistry to detect neutrophil elastase was performed on paraffin-embedded lung tissue sections from mice at 6 and 8 weeks of age. Shown are representative images from each group of at least six similar results