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Fig. 6 | BMC Immunology

Fig. 6

From: The impact of neutrophil extracellular trap from patients with systemic lupus erythematosus on the viability, CD11b expression and oxidative burst of healthy neutrophils

Fig. 6

The effect of NET on the respiratory burst of neutrophils. a Following treatments with or without NET, PMA-stimulated granulocytes were stained by DHR and run on the flow cytometer to determine their OB capacity. The OB ability of the incubated neutrophils with either healthy and SLE NET was enhanced compared to the unincubated cells; the difference between the the two latter groups was also significant. The OB capacity of neutrophils did not change with NET pretreatment alone (with no stimulation). b The mean fluorescence of the activated neutrophils stained by DHR (details in the Methods section) increased because of ROS production as a result of OB. The plot shows the overlaid histograms of the unstimulated, PMA-stimulated, NET-treated unstimulated, normal-NET-treated PMA-stimulated, and SLE-NET-treated PMA-stimulated neutrophils from the same sample plus their corresponding OBI (Oxidative Burst Index) in the table. The unstained neutrophils are also shown. NCM or NET control medium refers to the medium that was collected from the unstimulated healthy and SLE neutrophils in the NET-inducing experiments

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