Fig. 3

Influence of recombinant human CD100 stimulation to cytokine production and perforin/granzyme B secretion by CD8⁺ T cells from controls (n = 10) and acute myocardial infarction (AMI) patients (n = 10). CD8⁺ T cells were stimulated with recombinant human CD100 for 24 h. Cells and supernatants were then harvested. a IFN-γ and b TNF-α level in the supernatants was measured by ELISA, and was compared between controls and AMI patients, and between presence and absence of CD100 stimulation. c Perforin and d granzyme B production by CD8⁺ T cells was measured by ELISPOT. The number of spot-forming cells (SFC) was compared between controls and AMI patients, and between presence and absence of CD100 stimulation. Individual level for each subject was shown. The horizon lines indicated means, while the error bars indicated standard deviations. One-way ANOVA and SNK-q test was used for statistical analysis