Fig. 2

Effect of activin A on the expression of ActRIIA on peripheral lymphocytes. a The expression of ActRIIA on CD4⁺ T lymphocyte. b The expression of ActRIIA on CD8⁺ T lymphocyte. c The expression of ActRIIA on CD19⁺ B lymphocyte. Total PBMCs were isolated from Kawasaki disease patients and healthy controls, stimulated with activin A (5 ng/ml) for 24 h in vitro. Cells were then harvested, stained and the expression of cell surface molecules ActRIIA was analyzed by flow cytometry. Individual level of each subject was shown. The horizon line presented mean, and error bar presented standard deviation. HC, Healthy controls group; KD, Kawasaki disease group: KD Act, Kawasaki disease with activin-A stimulated group. Representative FlowJo plots were shown in supplementary material Fig.S2