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Fig. 3 | BMC Immunology

Fig. 3

From: Activin a suppresses peripheral CD8+ T lymphocyte activity in acute-phase Kawasaki disease

Fig. 3

Effect of activin A on activation of peripheral lymphocytes in acute-phase Kawasaki disease. a The expression of CD25 on CD4+ T lymphocyte. b The expression of CD69 on CD4+ T lymphocyte. c The expression of CD25 on CD8+ T lymphocyte. d The expression of CD69 on CD8+ T lymphocyte. e The expression of CD25 on CD19+ B lymphocyte. f The expression of CD69 on CD19+ B lymphocyte. Total PBMCs were isolated from Kawasaki disease patients and healthy controls, stimulated with activin A (5 ng/ml) for 24 h in vitro. Cells were then harvested, stained and the expression of CD25 and CD69 were analyzed by flow cytometry. Individual level of each subject was shown. The horizon line presented mean, and error bar presented standard deviation. HC, Healthy controls group: KD, Kawasaki disease group: KD Act, Kawasaki disease with activin-A stimulated group. Representative FlowJo plots were shown in supplementary material Fig.S3

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