Fig. 4

ERK phosphorylation, NFκB p65 phosphorylation, and NFAT activity in Tim-3^−/+ NK and Tim-3^−/+ CD4⁺ T cells. a–b Representative flow cytometry plots and statistical analyses of ERK phosphorylation in Tim-3^−/+ NK and Tim-3^−/+ CD4⁺ T cells (n = 4). c–d Representative flow cytometry plots and statistical analyses of NFκB p65 phosphorylation in Tim-3^−/+ NK and Tim-3^−/+ CD4⁺ T cells (n = 4). e–f Representative flow cytometry plots and statistical analyses of NFAT activity in Tim-3^−/+ NK and Tim-3^−/+ CD4⁺ T cells (n = 4). All data analyzed using FacsDiva™ 8.0.3 (URL: www.bdbiosciences.com) and FlowJo™ 10.5.0 (URL: www.flowjo.com/flowjo-eula/). Wilcoxon matched-pairs signed rank tests (GraphPad Prism 9.0, URL: www.graphpad.com) were used to compare the two groups; ^*p < 0.05, ^**p < 0.01, ^***p < 0.001, ^****p < 0.0001