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Fig. 6 | BMC Immunology

Fig. 6

From: The inhibitory receptor Tim-3 fails to suppress IFN-γ production via the NFAT pathway in NK-cell, unlike that in CD4+ T cells

Fig. 6

Influence of ERK, NFκB p65, and NFAT inhibitors on IFN-γ production by NK and CD4+ T cells. a–b Representative flow cytometry plots and statistical analyses of IFN-γ production following treatment with PD98059 (ERK inhibitor), PDTC (NFκB p65 inhibitor), 480401-M (NFAT inhibitor), PD98059 + PDTC, or PD98059 + PDTC + 480401-M, in NK cells (n = 4). c–d Representative flow cytometry plots and statistical analyses of IFN-γ production following PD98059, PDTC, 480401-M, PD98059 + PDTC, or PD98059 + PDTC + 480401-M treatment in CD4+ T cells (n = 4). All data analyzed using FacsDiva™ 8.0.3 (URL: www.bdbiosciences.com) and FlowJo™ 10.5.0 (URL: www.flowjo.com/flowjo-eula/). Wilcoxon matched-pairs signed rank tests (GraphPad Prism 9.0, URL: www.graphpad.com) were used to compare the two groups; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001

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