Fig. 1

NK cell population increase in TREM2-TG mice. a Flow cytometry analysis data represent the surface expression of NK-specific receptors (NGK2A/C/E, Ly49C/F/H/I, Ly49D) in the spleen, BM, and liver of WT and TREM2-TG mice. b The percentage (top panel) and absolute number (bottom panel) of NK1.1⁺ cells in the spleen (N = 6), BM (N = 7), and liver (N = 8) of WT and TREM2-TG mice. Each symbol represents an individual mouse; horizontal lines indicate the mean. *P < 0.05, **P < 0.01 and ***P < 0.001 by Student’s t-test. c-e Representative tSNE plots show BM cell population of WT (left panel) and TG mice (middle panel) stained with TREM2/NK1.1/CD11b/CD3/TREM2 (c) or TREM2/CD122⁺/CD11b/CD3 specific antibodies (d). Each cluster represents a group of TREM2⁺ (Cluster1, 5), CD11b⁺ (Cluster 3,7), CD3⁺ (Cluster4, 8), NK1.1⁺ (Cluster2), and CD122⁺ (Cluster 6) cell population, respectively (c and d right panel). (c, right panel) Cluster2 (blue) represents NK1.1⁺ cells and cluster1 (red) represents TREM2+ cells (d, right panel). Red circles indicate the TREM2⁺ population on the NK1.1⁺ cells (c) or CD122⁺ cells (d). The histogram represents the TREM2⁺ population on the NK1.1⁺ cells (c, bottom) and TREM2⁺ population on the CD122⁺ cells (d, bottom) of TG and WT mice. e These tSNE plots showed CD3⁺, CD122⁺, NK1.1⁺, and TREM2⁺ cell population in the BMs of WT (left panel) and TG mice (right panel). Red circles indicate the CD3⁻CD122⁺NK1.1⁺TREM2⁺ population in BMs of TG mice. Each tSNE plot represents CD3⁺, CD122⁺, NK1.1⁺, TREM2⁺ cell population separately. f Dot plots represent that BM of TG mice (left, lower) and WT mice (left, upper), which were stained with TREM2+, CD122+, and NK1.1+ antibodies. Black circles indicate CD122 + NK1.1+ population in the TREM2+ population. The percentage (right) of TREM2+ CD122 + NK1.1+ cells in the BM (N = 3) of WT and TREM2-TG mice. tSNE plots and dot plots were analyzed using Flow jo software