Fig. 5

Pre-treatment with tryptase or chymase decreased Poly (I:C) induced IFN and PRR expression in HBECs. HBECs were pre-treated with tryptase or chymase followed by Poly (I:C) stimulation after 3 h. Gene expression was measured in cell lysate. Gene expression of IFN-β (a), IFN-lambda (b), TLR3 (c), MDA-5 (d) and RIG-I (e). Protein expression of TLR3 (f), MDA-5 (g) and RIG-I (h). Representative western blot of PRR protein expression at 24 h (i). Data are presented as mean ± SEM, n = 4 from 4 independent experiments. **P < 0.01 compared to respective control and ^#P < 0.05, ^##P < 0.05 compared to Poly (I:C) alone