Fig. 3

SR9009 attenuated the polarization response of differentiated macrophages from U937 to LPS. A, The expression of Rev-erbα in differentiated macrophages from U937 with or without LPS treatment was analyzed by western blot. The displayed blots were cropped blots. Full-length blots were presented in Supplementary Fig. 2. B-E, Quantitation of flow cytometric analysis of the M1/M2 markers in differentiated macrophages from U937 stimulated by LPS in the presence or absence of SR9009 or PI3K inhibitor LY294002 (n = 6 independent experiments). F, Representation and quantitation of the protein level of TLR4 and total NF-κB and phosphorylation NF-κB in differentiated macrophages from U937 stimulated by LPS in the presence or absence of SR9009 or LY294002 (n = 3 independent experiments). The displayed blots were cropped blots. Full-length blots were presented in Supplementary Fig. 3. The samples derive from the same experiment and that blots were processed in parallel. G, ROS production detected by fluorescent probe DCFH-DA in differentiated macrophages from U937 stimulated by LPS in the presence or absence of SR9009 or LY294002. Data represented mean ± SEM of independent experiments. *P < 0.05, **P < 0.01, compared with control group without SR9009 and LY294002 treatment; ^#P < 0.01, ^###P < 0.001, compared with the group with LPS treatment; ^&P < 0.01, ^&&&P < 0.001, compared to the group with LPS and SR9009 treatment