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Fig. 6 | BMC Immunology

Fig. 6

From: Identification of active small-molecule modulators targeting the novel immune checkpoint VISTA

Fig. 6

The activity of compound 6809-0223 was detected by CBA in CD8+ T cells. Cells (1 × 105 cells/well) were incubated with an immobilized anti-mouse CD3 antibody (2.5 μg/mL) and mouse VISTA-ECD at 5 μg/mL, and compound 6809-0223 was added as indicated. The levels of a IL-2, b IL-4, c IFN-γ, d TNF-α in the cell culture supernatants were measured at 48 h with Cytometric Bead Array (CBA) Kits (n = 3)

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