Fig. 2

Humoral immune response. Humoral response was assessed 1 week before and 2 and 4 weeks after the booster vaccination. The results from commercially available ELISA kits are presented as the mean ± SEM (U/mL) in the graphs. The experiment was performed using five mice per group in the naïve groups and six mice per group 1 week before vaccination. In all other conditions, experiments were performed on 10 mice per group. Statistical differences were tested with two-way ANOVA and Tukey’s multiple comparison test. A IgG responses to diphtheria and tetanus were assessed by using commercially available ELISA kits. B Anti-PT, anti-FHA, and anti-PRN IgG titer levels were assessed by using commercially available kits. (SEM = standard errors of the means, PT = pertussis toxin, FHA = filamentous haemagglutinin, PRN = pertactin) (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001)