Figure 4

Analysis of MDP-specific CTL activity against CRC cell lines. Standard ⁵¹Cr-release cytotoxicity assays were performed with MDP05-specific CTL using CRC cell lines as target cells. Effector T cells were added at different effector to target cell (E:T) ratios. A representative experiment of CTL derived from (A) donor 1 and (B) donor 2 is shown. Analysis was performed in triplicates with 1.000 effector cells per well and after a 6 h incubation period. The expression levels of MDR-1 and MDR-3 are given as additional information (right upper corner) for untreated SW480 and SW707 and for cisplatin and 5-FU pretreated SW480 in copies/μl cDNA normalized to the housekeeping genes cyclophilin B and hypoxanthine guanine phosphoribosyltransferase.