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Figure 1 | BMC Immunology

Figure 1

From: Blomia tropicalis Blo t 5 and Blo t 21 recombinant allergens might confer higher specificity to serodiagnostic assays than whole mite extract

Figure 1

Expression and purification of recombinant proteins rBlo t 5 and rBlo t 21. The his-tagged proteins rBlo t 5 (A) and rBlo t 21 (B) were induced by IPTG and affinity-purified using the resin Ni-NTA agarose. The samples collected during expression induction and purification were analyzed by 15% SDS-PAGE stained with Coomassie Blue. Arrows indicate purified proteins. (C) Immunoblotting of the rBlo t 5 (A) and rBlo t 21 with negative control sera (Ns) and positive sera – asthmatic patient (Ps). MW, molecular weight.

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BMC Immunology

ISSN: 1471-2172

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