Figure 1From: Analysis of localized immune responses reveals presence of Th17 and Treg cells in cutaneous leishmaniasis due to Leishmania tropicaValidation of cDNA array results using real time PCR in tissue lesions of CL patients. Relative mRNA levels of IFN-γ, IL-10, MCP-1, IL-1β, IRF-1 and Fas were determined by QPCR in tissues lesions of CL patients (n = 10) or control tissues (n = 7). The relative quantification of products was determined by the number of cycles over endogenous control (β-actin mRNA) required to detect the gene expression of interest. Values of P were calculated using unpaired Student’s t test. Horizontal lines indicate mean values.Back to article page