Figure 2

Specific IgA (A) and IgG (B) anti Poli Vi antibody response in mice samples. 25 μg of Poli Vi (12.5 μg each nostril) was administered with or without PLc (50 μg each nostril) in BALB/c mice (n=5) by the intranasal route (10 μL per nostril). A three-dose schedule (0, 7 and 14 days) was used and samples were evaluated. Vax-TyVi vaccine was used as a positive control with only one dose by the i.m. route. (A): Seven days after the last immunization, saliva (white columns) and faeces (black columns) were collected and diluted 1:2. Results are expressed as a mean of optical density units (OD) ± standard deviation. (B): Fourteen days after the last immunization, sera was obtained and diluted 1:100. Results are expressed as a mean of optical density units (OD) ± standard deviation. A placebo group was also included in each experiment and the effect was subtracted for statistical analysis. Tukey test was used to analyze the data of two experiments. P values indicate significant differences between groups.