Groups | IgG(U/mL) sera | IgA(AU/mL) saliva | IgG1(OD) sera | IgG2a(OD) sera | IFNγ(pg/mL) |
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AFCo1 | 4415.74* | 2147.03* | 1.378* | 1.62* | 2787.04* |
Placebo | 106.05 | 101.48 | 0.102 | 0.120 | 162.381 |
PHA | - | - | - | - | 3065.34* |
- Groups of female BALB/c mice (n = 5) were immunized with AFCo1 by the i.n route (3 doses, 12.5µL in each nostril) with a 7 day interval. Anti-PLn IgA in saliva or serum and anti-PLn IgG and IgG subclass induction by AFCo1 and IFN-γ production by spleen cells from immunized mice. Phosphate Buffer was used for the Placebo group. IFN-γ concentration was evaluated in splenocyte supernatants, 7 days after the last dose and stimulated in vitro with PLn or phytohaemagglutinin (PHA). Asterisks denote significant differences between groups * p<0.05.