Figure 3
From: Flt3+ macrophage precursors commit sequentially to osteoclasts, dendritic cells and microglia
Macrophage differentiation in response to M-CSF (A) Day 6-FL cells were stained with May Grünwald Giemsa reagent after 4 days of culture in M-CSF (500 U/ml). (B) Phagocytic activity of M-CSF-differentiated day-6 FL cells is shown by ingestion of brown latex beads. (C) Flow cytometry analysis of CD11b, F4/80, CD34 and CD135 expression on M-CSF-differentiated day 6-FL cells. (D) Flow cytometry analysis of CD11b and F4/80 expression on M-CSF-differentiated day 6-, day 8-, and day 11-FL cells.