Nickel, palladium and rhodium induced IFN-gamma and IL-10 production as assessed by in vitro ELISpot-analysis in contact dermatitis patients

Bordignon, Valentina; Palamara, Francesca; Cordiali-Fei, Paola; Vento, Antonella; Aiello, Arianna; Picardo, Mauro; Ensoli, Fabrizio; Cristaudo, Antonio

doi:10.1186/1471-2172-9-19

Table 2 Individual values of spot forming cells for each experimental condition expressed as mean values and Standard Errors of triplicate wells.

From: Nickel, palladium and rhodium induced IFN-gamma and IL-10 production as assessed by in vitro ELISpot-analysis in contact dermatitis patients

			IFN-γ					IL-10
	Subject n.	Mean Std. Error	W/O	PHA	Ni	Pd	Rh	W/O	PHA	Ni	Pd	Rh
Patch test positive to Ni	1	Mean	18,67	151	116	14,33	5,667	19	132	36	79,67	76
(Group 1)		Std. Error	2,404	4,359	3,055	1,202	0,8819	0,5774	3,606	2,082	3,93	3,786
	2	Mean	25,17	164,3	150	29,33	17,67	21	157,3	36,5	73	86,33
		Std. Error	1,74	6,766	3,215	2,333	1,453	2,082	6,36	0,2887	2,309	2,333
	3	Mean	27,67	203	136,5	19,33	16,67	11,67	92	22,5	47	52
		Std. Error	2,333	3,606	0,866	0,8819	1,453	0,8819	3,606	1,443	3,606	2,887
	4	Mean	26,67	203	168,3	18,67	5,333	33,33	270	62,67	51,33	132,7
		Std. Error	2,404	9,074	3,48	1,202	1,202	1,202	6,351	6,333	1,667	5,207
	5	Mean	30,67	246,7	168,3	27,33	27,33	38	289	65,33	143,3	147,7
		Std. Error	0,8819	1,856	2,603	0,8819	1,764	2,517	6,083	2,603	2,963	7,311
	6	Mean	13,33	100,3	53	25	6,667	22	177	32,67	79,33	93,67
		Std. Error	2,186	3,18	3,055	1,732	1,202	5,568	6,658	2,906	0,8819	4,667
	7	Mean	21,33	171,7	119,3	10,83	10,5	21,67	147,3	35,67	85,33	91
		Std. Error	0,3333	3,667	4,667	0,6009	0,2887	2,848	5,925	3,283	2,603	2,082
	8	Mean	20	158	129,3	10,33	15,5	35	265,7	46,5	136	132,7
		Std. Error	1,155	3,464	3,18	1,764	1,443	2,309	11,98	0,866	6,245	5,487
	9	Mean	20,67	160,7	118,3	4,333	8,333	33,5	233	59	129,3	130
		Std. Error	1,764	5,239	4,41	1,453	1,453	0,866	7,506	2,887	5,783	3,215
	10	Mean	13,33	105,3	65	14,67	2,667	15,5	107,7	25,33	59	62
		Std. Error	2,404	5,696	2,082	1,453	1,202	1,607	3,844	3,844	2,082	1,732
Patch test positive to Pd	11	Mean	10	69,33	11	45,33	2,333	27	197,3	84,33	21	129,3
(Group 2)		Std. Error	1,155	3,48	0,5774	2,603	1,333	2,887	7,219	2,404	3,606	3,18
	12	Mean	14,33	100,3	20,67	83,67	15,67	16,5	124,7	58,67	10,33	68,33
		Std. Error	3,844	1,453	2,333	4,631	1,202	1,443	3,283	1,202	2,603	2,906
	13	Mean	13	92	19,33	62,33	14	37,67	285	143	38	186
		Std. Error	3,215	5,686	2,028	3,844	1,528	1,453	5,508	2,309	1,732	3,464
	14	Mean	13,5	101	17	71,67	10,67	35,5	285	127,7	30	151
		Std. Error	0,866	7,937	3,215	2,333	0,8819	0,2887	5,508	4,41	1,155	1,528
	15	Mean	45	360,3	41	229,7	36,33	9,333	73,67	37,67	10,67	41,33
		Std. Error	1,732	9,351	1,732	2,906	3,48	1,764	2,728	1,202	1,764	1,856
	16	Mean	6,5	53	5,667	34	2	12	92	47,33	13,67	45,33
		Std. Error	1,443	2,082	1,202	1,155	1,528	1,528	2	1,856	1,764	1,764
	17	Mean	15,67	117,3	9,667	72,67	6,333	23,33	190	84	22	93
		Std. Error	3,48	7,688	0,6667	1,453	0,8819	2,848	2,082	2,082	2,309	1,155
	18	Mean	6,667	52	5,333	32	2	28,67	198,3	110,3	24	112
		Std. Error	1,764	3,512	0,8819	2,082	1	1,856	10,4	9,171	1,155	7,371
	19	Mean	32,67	273	36	170,3	13,33	10,33	70,33	34,33	9,667	44
		Std. Error	1,202	3,606	2,646	5,548	1,202	0,8819	2,728	3,283	1,202	2,646
	20	Mean	30,67	244	24,33	153,3	7	28,67	197,3	120,7	31,67	116
		Std. Error	1,202	4,509	2,028	2,848	1,528	2,404	2,333	8,95	1,764	4,041
Patch test positive to Ni and Pd	21	Mean	14	105,7	89,67	17,67	10,67	20,33	153,7	32,67	72,33	81
(Group 3)		Std. Error	2,646	8,293	6,692	2,404	1,764	1,453	5,783	3,528	2,333	3,786
	22	Mean	24,83	172	150	25	25	22	176	26,67	79,67	93,67
		Std. Error	2,048	3,786	3,464	2,082	2,887	5,568	6,658	3,283	2,333	2,603
	23	Mean	26,67	203,7	157	37,33	14,67	38,67	292,3	66,67	148,7	152
		Std. Error	1,453	8,686	4,619	2,333	1,764	2,186	5,783	1,856	4,055	3,606
	24	Mean	31	247	186	30,67	18,33	23,67	186,3	21,33	94,67	119,3
		Std. Error	1,155	2,309	3,464	1,453	2,028	4,702	7,623	3,528	4,41	1,856
	25	Mean	25,67	197	157,7	22,33	18	37,33	285	49	145,7	143
		Std. Error	1,764	4,041	1,856	2,028	2,309	1,453	5,508	3,215	3,383	3,215
	26	Mean	20	157,7	117	16	10	15,5	125,3	29,67	56	61,67
		Std. Error	1,155	3,18	2,082	1,155	2,517	0,2887	4,667	1,667	2,887	1,764
	27	Mean	12,33	95,67	70,33	12	9,667	30,33	210,7	39,67	114,7	118,3
		Std. Error	1,453	10,37	3,383	1,528	2,028	3,18	6,984	4,978	3,48	3,756
	28	Mean	45	361	244,3	49,67	52,67	31,67	215	50	124,7	133
		Std. Error	1,732	9,292	4,096	3,283	2,333	2,963	5,132	2,646	3,48	2,309
	29	Mean	45,5	373,3	285	60	40	38,33	264,3	72,67	161,7	153,3
		Std. Error	2,021	2,963	6,429	1,732	3,512	4,055	12,25	2,028	4,055	3,48
	30	Mean	15,67	118	96	6,333	16,33	33,67	254,3	54	132	131,3
		Std. Error	2,333	3,215	6,928	2,028	2,728	2,963	8,413	2,309	5,292	2,963
Patch test negative	31	Mean	3,667	24	6	4,667	4,667	32,33	243	146,7	116,7	134,3
(Group 4)		Std. Error	0,8819	1,528	1,155	0,8819	1,202	2,028	4,041	4,631	6,173	5,608
	32	Mean	17,33	130	27,67	21,33	33	47,33	377	237,3	181	201,3
		Std. Error	2,333	2,887	1,764	3,283	1,732	2,728	2,646	2,603	3,215	6,064
	33	Mean	6,667	53,33	10,33	4,333	5,667	18,33	140,3	69,67	66,33	90,67
		Std. Error	1,202	2,728	0,8819	1,453	0,6667	1,453	2,603	2,404	4,842	4,631
	34	Mean	11,33	82,67	15	8	8	24,33	193,3	98	99	108
		Std. Error	0,8819	2,333	2,082	1,732	1,528	1,202	3,844	1,732	4,619	5,196
	35	Mean	14,33	112,7	17,33	18,67	7,333	34,33	260,3	134	111,7	132
		Std. Error	1,202	5,044	1,453	2,028	0,8819	2,404	3,18	1,528	4,41	4,359
	36	Mean	3	23	3,333	0,6667	2,333	17	138	78,33	83	67,67
		Std. Error	0,5774	1,528	1,453	0,6667	0,3333	2,646	4,163	1,453	3,055	2,404
	37	Mean	18	141	23,33	7,333	4,333	15	109,7	57,33	57,33	58,67
		Std. Error	1,732	5,033	1,453	1,202	0,8819	1,732	2,906	2,404	3,18	4,256
	38	Mean	22,67	180,7	27,33	18,33	11,67	29	196,7	154,3	114,3	122,7
		Std. Error	1,202	1,202	2,404	1,453	1,202	1,732	1,453	2,404	6,009	4,41
	39	Mean	6,667	54	6,333	2	5,333	10	71,33	52,67	40,33	40,33
		Std. Error	1,202	2	0,8819	1,155	1,856	1,155	2,028	2,028	2,906	4,096
	40	Mean	13,33	105,3	10,67	11	15	27,67	199,3	146,3	107,7	107
		Std. Error	1,453	3,712	0,8819	1,528	1,528	1,453	3,756	11,26	4,978	7

Patients were grouped following their patch test reactions. W/O: medium alone; PHA: polyclonal stimulation; Ni 20 μg/ml, Pd 2.5 μg/ml, Rh 5 μg/ml.

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